High concentration sonoperfused cultures of recombinant cho cells using a new generation of microcarriers with 2D geometry: process validation

Project Information

Grant agreement ID: BIO4979087

Project closed

Start date 24 June 1997

End date 15 October 1997

Funded under

• Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

Computer Cell Culture Center SA
Belgium

Objective

The low surface area-to-volume ratio offered by non-macroporous microbeads (microsupport with 3D geometry) to anchorage-dependent cells to grow into, makes it very difficult to reach cell concentration in excess of 5x10E6 cells/ml. The proposed Demonstration aims at validating at a cell concentration of 2,5x10E7 cells/ml NUNC's new generation of microsupport characterized by 2D geometry in 8L continuously stirred tank reactors. (Then also 20L). Supplementation with fresh nutrilites and simultaneous elimination of the toxics accumulated in these high cell density culture will be realized by sonoperfusion using the acoustic resonating wave technology developed by Anton PAAR GmbH. Validation of the latter acoustic separator (PAS) constitutes the other aim of the proposed Demonstration.

Fields of science

• natural sciences › mathematics › pure mathematics › geometry

Programme(s)

• FP4-BIOTECH 2 - Specific research, technological development and demonstration programme in the field of biotechnology, 1994-1998

Topic(s)

• 01 - Cell factories

Call for proposal

Data not available

Funding Scheme

EAW - Exploratory awards

Coordinator

Computer Cell Culture Center SA

• EU contribution: No data
• Address: 20,Place du Parc ; 7000 Mons ; Belgium
• Total cost: No data

Participants (2)

Anton Paar GmbH

Austria

• EU contribution: No data
• Address: 322,Kdrtner Strasse ; 8054 Graz
• Total cost: No data

Nunc A/S

Denmark

• EU contribution: No data
• Address: 90,Kamstrupvej ; 4000 Roskilde
• Total cost: No data