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High concentration sonoperfused cultures of recombinant cho cells using a new generation of microcarriers with 2D geometry: process validation

Objective



The low surface area-to-volume ratio offered by non-macroporous microbeads (microsupport with 3D geometry) to anchorage-dependent cells to grow into, makes it very difficult to reach cell concentration in excess of 5x10E6 cells/ml. The proposed Demonstration aims at validating at a cell concentration of 2,5x10E7 cells/ml NUNC's new generation of microsupport characterized by 2D geometry in 8L continuously stirred tank reactors. (Then also 20L). Supplementation with fresh nutrilites and simultaneous elimination of the toxics accumulated in these high cell density culture will be realized by sonoperfusion using the acoustic resonating wave technology developed by Anton PAAR GmbH. Validation of the latter acoustic separator (PAS) constitutes the other aim of the proposed Demonstration.

Funding Scheme

EAW - Exploratory awards

Coordinator

Computer Cell Culture Center SA
Address
20,Place Du Parc
7000 Mons
Belgium

Participants (2)

Anton Paar GmbH
Austria
Address
322,Kdrtner Strasse
8054 Graz
Nunc A/S
Denmark
Address
90,Kamstrupvej
4000 Roskilde