Objective
Modification of the flow of metabolic pathways by the genetic engineering of regulatory genes coding for transcription factors controlling the expression of the enzymes of these pathways.
The programme objective is to identify genes regulating metabolic pathways of nitrate assimilation and anthocyanin biosynthesis and to isolate and characterize clones of transcription factors involved in these processes.
During the first year several new regulatory mutans affecting anthocyanin biosynthesis have been identified. Simultaneously different genes coding for transcription factors are being cloned in the contracting laboratories deoxyribonucleic acid (DNA) retardation experiments restriction fragment length polymorphism, (RLP analysis), mutant complementation, and antisense strategies will be used as tools to identify the fuctions of these newly identified genes. Efforts will now be put on the identification of their targets and a major breakthrough with respect to the identification of their function is expected.
Many metabolic pathways can contribute to the quality of a crop. Some of these metabolic pathways lead to the undesirable accumulation of toxic compounds, whereas other pathways play a key function in biomass production or the expression of desirable characteristics. The purpose of the research project will be to explore the possibility of modifying the activity of such metabolic pathways by genetic engineering. A classical approach involves the identification of enzymatic steps limiting the flux of metabolites and their modification by genetic engineering. Since the non-coordinated modification of a metabolic step might reveal other limiting steps, or result in the over-accumulation of undesirable metabolites, our approach will be to identify and isolate genes specifically regulating metabolic pathways and to use genetic engineering to modify coordinately the tuning of the metabolic steps involved in these metabolic pathways. Three pathways have been chosen to develop this work: The flavonoid biosynthetic pathway, the carbon fixation pathway and the nitrogen assimilatory pathway.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology environmental engineering energy and fuels biomass energy
- natural sciences biological sciences genetics DNA
- medical and health sciences medical biotechnology genetic engineering
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
78026 VERSAILLES
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.