Objetivo
General objectives are:
to bring non-conventional yeast species to be genetically manipulatable organisms;
to develop expertise for the control of their physiology;
to create new combinations of genetic elements suitable for the production of medical and agricultural interest.
From a newly discovered plasmid pKD1, stable, high copy number vectors have been constructed. The system allows cloning and expression of genes in Kluyveromyces lactis and related species. In a model system, secreted production of a human protein has been made. This is the first stable vector system available for the biotechnological manipulation of the lactic yeast Kluyveromyces.
Research is progressing on the line defined objectives. Mapping of genes K lactis has progressed by construction and use of multiply labelled strains and centromere linked markers. Data on physical mapping on chromosomes of cloned genes are accumulating in parallel, confirming genetic data. To support mapping work, many new mutations were isolated and several genes were cloned;
4 genes encoding alcohol dehydrogenase isozymes have been identified by hybridiztion to ADH2 probes of S cerevisiae. The genes have been isolated and sequenced;
Chromosomal deoxyribonucleic acid (DNA) fragments from K lactis have been tested for the ability of efficiently sustaining autonomous replication of plasmids in K lactis or in S cerevisiae. Some of these fragments have ARS function only in K lactis while others support autonomous replication in both yeasts;
Studies on the strong promoter of the methanol oxidase gene in Hansenula polymorpha have revealed a complex mode of regulation;
In Kluyveromyces lactis, the detailed knowledge on the structure and mode of regulation of the beta-galactosidase (LAC4) promoter is being exploited to establish expression systems for heterologous genes;
The resistance of different strains of Yarrowia lipolytica was analyzed and copper sensitive mutants have been obtained. Using an integrative bank of Yarrowia lipolytica it has been possible to complement one of the mutations;
4 clones of stress inducible genes isolated from Saccharomyces cerevisiae (HSP12, HSP26, ESP30 and ESP35) were used as hybridization probes for a Southern blot of analysis of K lactis genomic DNA. Clones hybridizing with these probes (except HSP26) could be isolated. It has not been necessary to construct complementary deoxyribonucleic acid (DNA) libraries for their isolation;
Many new mutants of Kluyveromyces lactis affected in carbohydrate metabolism were isolated. The genetic analysis demonstrated that the isolated mutants define several complementation groups;
Ka ryotyping of wild type Y lipolytica isolates revealed a pronounced diversity of patterns and individual gene probes showed that gross chromosomal rearrangements are associated with chromosomal polymorphism;
Work has started on the construction of recombinant Kluyveromyces lactis strains harboring expression cassettes either integrated at a defined chromosomal locus (RAG2) or contained on multi-copy vectors derived from the nature Kluyveromyces plasmid pKD1.
The fermentation characteristics of Kluyveromyces marxianus have been studied. The fermentation process is aiming at a very high cell density and high production/secretion of the homologous enzyme inulinase.
The proposal involves 7 academic institutions and 3 companies. The existing 400 species of yeast, represented by a large number of strains in the worldwide collection centres, are a great resource for scientific and industrial exploitation. Yeast can be used in two kinds of production:(i) primary metabolites and derivatives, (ii) homologous and heterologous proteins. In the first category, non-conventional species have already been used mostly on the basis of classical technology of physiological regulation. In the second category where the products are of high added value, recombinant DNA techniques are the essential tool. Therefore, recent attempts for the latter type of production have been made almost exclusively with Saccharomyces in which gene transfer systems are well established, while other species are only at the beginning of such molecular approaches. The experience with Saccharomyces and recent results with several non-conventional species have shown the limitations of the Saccharomyces system and the new possibilities offered by other species, in particular, Kluyveromyces, Hansenula/pichia and Yarrowia, for protein production. The major objectives of the proposal are:(i) to bring these species to be genetically manipulatable organisms, (ii) to develop expertise for the control of their physiology, and (iii) to create new combinations of genetic elements suitable for the production of proteins of medical and agricultural interest.
Ámbito científico (EuroSciVoc)
CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural. Véas: El vocabulario científico europeo..
CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural. Véas: El vocabulario científico europeo..
- ciencias naturales ciencias biológicas genética ADN
- ciencias naturales ciencias biológicas genética mutación
- ciencias naturales ciencias biológicas bioquímica biomoléculas carbohidratos
- ciencias naturales ciencias químicas química orgánica alcoholes
- ciencias naturales ciencias biológicas bioquímica biomoléculas proteínas enzima
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Programas de financiación plurianuales que definen las prioridades de la UE en materia de investigación e innovación.
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Las convocatorias de propuestas se dividen en temas. Un tema define una materia o área específica para la que los solicitantes pueden presentar propuestas. La descripción de un tema comprende su alcance específico y la repercusión prevista del proyecto financiado.
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Procedimiento para invitar a los solicitantes a presentar propuestas de proyectos con el objetivo de obtener financiación de la UE.
Régimen de financiación
Régimen de financiación (o «Tipo de acción») dentro de un programa con características comunes. Especifica: el alcance de lo que se financia; el porcentaje de reembolso; los criterios específicos de evaluación para optar a la financiación; y el uso de formas simplificadas de costes como los importes a tanto alzado.
Régimen de financiación (o «Tipo de acción») dentro de un programa con características comunes. Especifica: el alcance de lo que se financia; el porcentaje de reembolso; los criterios específicos de evaluación para optar a la financiación; y el uso de formas simplificadas de costes como los importes a tanto alzado.
Coordinador
91405 Orsay
Francia
Los costes totales en que ha incurrido esta organización para participar en el proyecto, incluidos los costes directos e indirectos. Este importe es un subconjunto del presupuesto total del proyecto.