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The European image consortium for integrated molecular analysis of human gene transcripts

Objective

- To generate a minimal set of non redundant cDNA clones for most human gene transcripts and a master set of unique full-length cDNA clones representing 3,000 transcripts based upon the IMAGE Consortium resources (arrays of cDNA and CpG islands clones).
- To characterize by DNA sequencing with high accuracy the complete sequence of the master set of 3,000 human gene transcripts (6 Mbases of finished sequence).
- To obtain high resolution and comparative functional mappig localization in man and model organisms of 1,000 genes represented in the master set.
- To develop the IMAGE Consortium Data Base to provide an easy access to an integrated view of the sequence, map and expression data generated.

The general objectives of the European IMAGE Consortium are:
- To generate a miniminal set of non redundant cDNA clones for most human gene transcripts and a master set of unique full-length cDNA clones representing 5,000 transcripts based upon the IMAGE Consortium resources (arrays of cDNA and CpG islands clones);
- To characterize by DNA sequencing with high accuracy the complete sequence of the master set of 5,000 human gene transcripts (10 Mbases of finished sequence);
- To identify positional and functional candidate genes involved in specific biological functions and inherited human diseases through high resolution and comparative functional mapping in man and model organisms of 1,000 genes represented in the master set;
- To explore the transcription profiles of the genes represented in the minimal and master sets, and to produce 800 of the cognate proteins in order to explore their function;
- To develop the IMAGE Consortium Data Base to provide an easy access to an integrated view of the sequence, map and expression data generated.

The European IMAGE Consortium will work to consolidate the achievements of the Consortium on cDNA Resources for Physical Mapping. 20 % of the resources will be devoted to the assembly of the physical resources (cDNA and CpG island arrays characterized by end sequencing and fingerprinting, minimal sets of clones selected after comprehensive sequence, clone and functional clustering, master set of 5,000 full-length clones). These resources will be available throughout the European Union for the user community in both academy and industry. The Consortium will serve the future needs of the scientific community in the systematic identification of all human genes and the irregulatory sequences by deciphering in an efficient and economic manner 10 Mb of complete, finished sequence for 5,000 transcripts of average size 2 kb: 50% of the resources will be devoted to the sequencing of full-length cDNAs and CpG islands selected by Consortium members and other EU-sponsored projects on the basis of their map position, similarity to known families or expression profiles.

In order to ensure that advances in basic genetic knowledge is used to further enhance human health, the Consortium will seek to contribute to the identification of genes involved in human biology and diseases by correlating precise map location and phenotypic expression data, exploiting various comparative approaches for 1,000 of the genes represented in the master set: 10% of the resources will be devoted to high-resolution and comparative functional mapping in close interaction with the mapping consortia in order to obtain the most precise and evolutionary relevant map location; 10% of the resources will be devoted to the collection of transcription profiles of the genes represented in the minimal as master sets, using high density array hybridisation, and to the exploration of methods for systematic protein production, as means to progress toward the identification of the function of 800 of the cognate proteins. Last but not least, 10 % of the resources will be devoted to the IMAGE Consortium Data Base. This will provide the community with up to date, integrated sequence, mapping and expression data related to the IMAGE consortium arrays, as they are collected by Consortium members in Europe, the United States and Japan, and will help in sharing and harmonizing suchdata. The European IMAGE Consortium objectives comply with the objectives of the BIOMED 2 Program and priority research tasks under Section 5 (Human Genome Research), with particular emphasis on subsection 5.1 (Gene mapping) and 5.5(Information management and analysis).

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
Address
Rue Guy Môquet 19
94801 Villejuif
France

Participants (7)

CANCER RESEARCH INSTITUTE
Spain
Address
Km 2,7,Gran Via S/n, Km 2,7
08907 Hospitalet Del Llobregat
EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany
Address
Meyerhofstrasse 1
69117 Heidelberg
FONDAZIONE TELETHON
Italy
Address
Via Pietro Castellino 111
80131 Napoli
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Germany
Address
73,Ihnestraße 73
14195 Berlin
MEDICAL RESEARCH COUNCIL
United Kingdom
Address
Babraham Bioincubator
CB2 4AT Cambridge
ROYAL INSTITUTE OF TECHNOLOGY
Sweden
Address
34,Roslagstullsbacker, 21
100 44 Stockholm
Stiftung Deutsches Krebsforschungszentrum
Germany
Address
280,Neuenheimer Feld
69120 Heidelberg