Identification and Microassay of Neuroactive Steroids : Physiological and Pathophysiological Significance

Información del proyecto

Identificador del acuerdo de subvención: BMH4972349

Proyecto cerrado

Fecha de inicio 1 Julio 1997

Fecha de finalización 30 Junio 2000

Financiado con arreglo a

• Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998

Coste total Sin datos

Aportación de la UE Sin datos

Coordinado por

Institut National de la Santé et de la Recherche Médicale
France

Objetivo

To set up a centralized facility in Europe for the microassay of steroids by gas-chromatography-mass fragmentography (GC-MF), operating the MF in the negative ion chemical detection (NICI) mode.
To compare individual characteristics and performances with the concentrations of relevant steroids in defined nervous structures, and thus to establish the physiological and pathophysiological role of neurosteroids in nervous system functioning.

The specific aim of this proposal is to measure neurosteroids in both CNS and PNS, under normal and pathological conditions and after injury by GC-MF. The steroids to be measured are those with documented neuroactive properties : either steroid hormones involved in genomic regulation (progesterone, 5a-dihydroprogesterone, deoxycorticosterone, corticosterone or cortisol, testosterone and 5a-dihydrotestosterone), or steroids endowed with neuromodulatory properties (allopregnanolone, tetrahydrodeoxycorticosterone, pregnenolone sulfate, dehydroepiandrosterone sulfate). A complete validation of this new method as applied to neurosteroids will be needed.
Steroid assays will also be performed in cell culture and coculture media and will be completed by biochemical and biological molecular investigations of the enzymes involved in their biosynthesis and metabolism.
Interest will focus on the in vitro and in vivo trophic effects of neurosteroids, as involved in nerve regeneration and myelin repair, and on the behavioural effects of neurosteroids, particularly on ageing related memory disturbances.

Ámbito científico (EuroSciVoc)

CORDIS clasifica los proyectos con EuroSciVoc, una taxonomía plurilingüe de ámbitos científicos, mediante un proceso semiautomático basado en técnicas de procesamiento del lenguaje natural.

• ciencias naturales › ciencias biológicas › neurobiología
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas › enzima

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Programa(s)

• FP4-BIOMED 2 - Specific research, technological development and demonstration programme in the field of biomedicine and health, 1994-1998

Tema(s)

• 3.1 - Pathophysiology and basic mechanisms

Convocatoria de propuestas

Data not available

Régimen de financiación

CON - Coordination of research actions

Coordinador

Institut National de la Santé et de la Recherche Médicale

• Aportación de la UE: Sin datos
• Dirección: 80,Rue du General Leclerc ; 94276 KREMLIN-BICETRE ; Francia
• Coste total: Sin datos