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A transcript map of the mouse genome by radiation hybrid mapping

Objective

- To establish an expressed sequence tag based transcript map of the mouse genome.

This project aims to make a major contribution to the establishment of a transcript map of the mouse genome. Expressed sequence tags (ESTs) will be generated and mapped on a specially constructed hybrid (RH) panel on which framework markers will have been localised. Specific attention will be paid to including markers which will allow integration of this map with other mouse mapping resources. We will exploit the strengths of the mouse system by concentrating on generating and mapping ESTs from stages of mouse development difficult to study in humans such as gastrulation which is known to involve high complexity transcription. Parallel expression profile analysis of a subset of the mapped transcripts will be integrated with functional analysis based on targeted mutagenesis. The workplan takes advantage of the participants' expertise in mouse embryology, genetics and high throughput genomic analysis. The transcript map will improve the utility of the mouse as a model system for human disease and biology by generating candidate genes for the 1500 known mouse mutant phenotypes, for the new mutants being generated in current ENU mutagenesis programmes and new targets for directed mutational approaches.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

GENOSCOPE-CENTRE NATIONAL DE SÉQUENÇAGE
Address
Rue Gaston Crémieux 2
91057 Evry
France

Participants (4)

ISTITUTO NAZIONALE PER LO STUDIO E LA CURA DEI TUMORI
Italy
Address
Via Venezian 1
20133 Milano
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.
Germany
Address
73,Ihnestraße 73
14195 Berlin
MEDICAL RESEARCH COUNCIL
United Kingdom
Address
Harwell
OX11 0RD Didcot,harwell,chilton
MRC National Institute for Medical Research
United Kingdom
Address
The Ridgeway Mill Hill
NW7 1AA London