1. Capillary tubes made of Polymethylpentene with good binding properties and low background fluorescence. Transparency and surface smoothness acceptable but not of optical quality.
2. Development of biocompatible photoresist and lithographic processes for wide use patterning of biomolecules through the lift-off technique.
3. Development of photo grafting patterning of the capillaries using aminogroup photoreagents.
4. Development of discrete capillary bands by syringe injection of biomolecules.
5. Complete capillary multiband immunosensor tested against clinical and environmental analytes.
A new type of an optical immunosensor that holds the promise of inexpensive fast and sensitive multianalyte assays is proposed. It can be used for both clinical and environmental applications. The devices are based on the capillary geometry and are made of
inexpensive materials such as polystyrene, polymethylmethacryiate (PMMA) or polycarbonate. The inner surface of the capillaries is properly functionalized through biological and photochemical techniques to allow coating of different bands with different biomolecules. The capillary geometry facilitates such a partition which provides for multianalyte determinations. The multianalyte feature is a major point of this proposal since the simultaneous determination of relevant analytes cuts assay cost and time, while the capillary geometry substantially suppresses the required reagent and sample volumes.
Another major point of this proposal is the function of the capillary as an optical fiber guiding the light emitted by each band to its ends where a detector is placed. The instrumentation required for excitation and detection is quite simple. It consists of a pulsed light source of appropriate wavelength and a silicon detector followed by the low noise analog front end electronics and a digitizer. In conclusion, the proposal aims at adding value to inexpensive polymers through proper geometry and surface functionalization so that they can be used as immunosensors in large scale clinical and environmental applications.
Funding SchemeCSC - Cost-sharing contracts
9747 AG Groningen
RG12 6ET Bracknell