CORDIS provides links to public deliverables and publications of HORIZON projects.
Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .
Deliverables
Capillary tubes from Polymethylpentene were produced from a suitable laboratory extruder. Several different extrusion process parameters were tried, and using the best possible combination, capillary tubes were obtained having characteristics suitable for use in the field of immunoassays for fast determinations using minimal amount of reagents and with simplified sample handling and elimination of pipetting.
The suitability mainly refers to good protein binding properties and low background fluorescence. The background fluorescence sets the sensitivity limit of any optical biosensor and, therefore, any new material and format with reduced fluorescence can have broad applications. The capillaries were internally functionalised by immobilizing different proteins in discrete bands so as to create multi-Analyte biosensing devices.
Tests using environmental and clinical analytes confirmed the ability for fast detection with minimal use of reagents. Market or application sectors include human diagnostics, veterinarian diagnostics, food contamination control and environmental diagnostics. Further improvement is required to produce optically perfect devices.
A novel, biocompatible photoresist and process for the selective biomolecular functionality of solid support has been developed. This lift-off approach to patterning of biomolecules has the advantage of being independent from the specific biomolecule. The photoresist requires relatively low exposure doses, low baking temperatures and is developed in developers that will not affect the biomolecules.
It has already been tried for the inner partition of capillaries where the photolithographic requirements are even more demanding, owing to the cylindrical geometry and the results were satisfactory. At planar surfaces performs even better. It can be used for a wide range of applications requiring patterning of protein or DNA molecules, such as biosensor arrays.
Further development work is needed for achieving wider process windows. Additionally, photo-grafting techniques have been tried for the patterning of capillaries using amino group carrying photo reagents with encouraging results.
Exploitable results
Capillary tubes from Polymethylpentene were produced from a suitable laboratory extruder. Several different extrusion process parameters were tried and using the best possible combination capillary tubes were obtained having characteristics suitable for use in the field of immunoassays for fast determinations using minimal amount of reagents and with simplified sample handling and elimination of pipetting. The suitability mainly refers to good protein binding properties and low background fluorescence. The background fluorescence sets the sensitivity limit of any optical biosensor and, therefore, any new material and format with reduced fluorescence can have broad applications. The capillaries were internally functionalized by immobilizing different proteins in discrete bands so as to create a multi-Analyte biosensing devices. Testing using environmental and clinical analytes confirmed the ability for fast detection with minimal use of reagents. Market or application sectors include human diagnostics, veterinarian diagnostics, food contamination control and environmental diagnostics. Further improvement is required to produce optically "perfect" devices.
To exploit the benefits in terms of assay speed, reagent volumes, simplified sample handling and elimination of pipetting, a capillary immunoanalyzer has been in principle designed. It consists of 4 compartments, including capillary storage, capillary transfer, sample management and detection compartments. A belt based transfer system moves the capillaries across the various compartments with the help of a stepping motor. Automatic valves connect to the capillary ends, for sample reagent and washing solutions aspiration and dispensing using motorized dispensing syringes. Owing to the multi-analyte nature of the capillaries and their small volume, the immunoanalyzer can have the footprint of a A4 paper and could be ideal for clinical uses where the speed increase and the cost reduction of the assays are critical factors for improved public health care management.
A novel, biocompatible photoresist and process for the selective biomolecular functionalization of solid support has been developed. This lift-off approach to patterning of biomolecules has the advantage of being independent from the specific biomolecule. The photoresist requires relatively low exposure doses, low baking temperature and is developed in developers that will not affect the biomolecules. It has already been tried for the inner partition of capillaries where the photolithographic requirements are even more demanding, owing to the cylindrical geometry and the results were satisfactory. At planar surfaces performs even better. It can bee used for a wide range of applications requiring patterning of protein or DNA molecules, such as biosensor arrays. Further development work is needed for achieving wider process windows. Additionally, photografting techniques have been tried for the patterning of capillaries using aminogroup carrying photoreagents with encouraging results.
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