Objective
STUDIES ON THE CELLULOLYTIC SYSTEM OF CLOSTRIDIUM CELLULOLYTICUM WERE BROACHED. THE PRODUCTION OF THE MAIN MEASURABLE ACTIVITIES (CMCASE, PNPCBASE, AVICELASE) WAS FOLLOWED DURING GROWTH ON CELLULOSE. FOR ALL MEASURED ACTIVITIES, THE PRODUCTION WAS FOUND GROWTH DEPENDENT. ENZYMES RESPONSIBLE FOR PNPCBASE ACTIVITY WERE RELEASED ONLY POORLY IN THE SUPERNATANT. IN OPPOSITE, 30% OF THE CMCASE ACTIVITY WAS FOUND SOLUBLE AT THE END OF THE CULTURE.
TENTATIVES TO SEPARATE THE VARIOUS ACTIVITIES BY CLASSICAL CHROMATOGRAPHIC TECHNIQUES WERE DONE. 4 ACTIVES FRACTIONS (A,B,C,D) ON CMC WERE SEPARATED USING DEAE TRISACRYL. THE MAIN ACTIVE (B) WAS FURTHER SEPARATED IN TWO OTHER ACTIVE FRACTIONS (B1,B2) BY GEL FILTRATION USING ULTROGEL AC4-34. A RELATIVE INCREASE IN SPECIFIC ACTIVITY WAS OBTAINED BUT ANALYSE BY POLYACRYLAMIDE GEL ELECTROPHORESIS IN NON DENATURING CONDITIONS SHOWED A REMAINING NON HOMOGENEITY IN THE FINAL FRACTIONS OBTAINED.
TWO GENES, PB 52 AND PB 43, CODING FOR TWO ENDOGLUCANASES OF C.CELLULOLYTICUM WERE SUBCLONED FROM PACYC 184 PLASMID OF A GENE BANK INTO PLASMIDS PUC 18 AND PUC 19. THE RESTRICTION MAPS WERE DONE. BOTH GENES CAN BE EXPRESSED IN E.COLI INDEPENDENTLY OF THEIR ORIENTATION. THE PRODUCTS OF THESE GENES WERE ESSENTIALLY LOCALIZED IN THE CYTOPLASMIC COMPARTMENT OF E.COLI.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences electrochemistry electrophoresis
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
13402 Marseille
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.