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Protocol for the evaluation of residues in industrial contaminated liquid effluents

Deliverables

Summary: PERICLES' have been able to develop and evaluate a protocol for an integrated ecotoxicological/chemical assessment of industrial liquid effluents, using advanced ecotoxicological assays and analytic methods. The developed protocol represents a new improved tool to control industrial effluents. Within this project, simple and cost-effective ecotoxicological biofests have been studied in parallel with conventional ecotocixity tests. Different biotests have been combined in a more powerful indication of potential adverse effects of the effluents. The typology of substances identified confirms the complementarity of information which can be obtained with the different techniques. Reproducibility of qualitative analyses with GC-MS technique was confirmed. The main goal to identify chemicals responsible for the observed toxicity have been verified in effluents of different origin, including samples from Industrial waste landfills and a textile facility efflluent. Experimental and literature toxicity data have been used to theoretically explain toxicity of samples on the basis of quantitative analyses. We obtained a list of pollutants positively identified with standards, and characterized with toxicity data. Toxicity of the samples have been experimentally confirmed with reconstructed mixtures of samples mimicking real samples. A Parallel Quantitation of monyphenols in a textile effluent has been experimentally confirmed with reconstructed mixtures of samples mimicking real samples. These pollutants are very important because of their activity as endocrine disruptors.

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