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Development of new multisensing biosensors for the detection of bioavailable heavy metals in solid matrices.

Objective



The following research tasks will be carried out during the project:
1) Design of regulatory cascades and funnels for the simultaneous and specific detection of metal ions.
2) Development of bifunctional reporter systems.
3) Cloning of metal responsive DNA sequences in specialised vectors and expression into A. eutrophus.
4) Overexpression, purification, characterisation of specific and non-specific metal binding proteins.
5) Construction and properties of one gene-based (multisensing) and one protein-based prototype.
6) Testing and evaluation of the biosensors properties on chemically pre-analyzed solid wastes (soils, flyashes, sludges) in lab and field conditions.
7) Comparison of the chemical data and the data generated by the biosensor devices. Inter-laboratory tests.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

VITO - Vlaamse Instelling voor Technologisch Onderzoek NV
Address
200,Boeretang
2400 Mol
Belgium

Participants (5)

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Address
144,Velazquez 144
28006 Madrid
LUND UNIVERSITY
Sweden
Address
60,Chemical Center
221 00 Lund
NATIONAL ENVIRONMENTAL RESEARCH INSTITUTE - MINISTRY OF ENVIRONMENT AND ENERGY
Denmark
Address
399,Frederiksborgvej 399
4000 Roskilde
Tauw Milieu BV
Netherlands
Address
11-13,Handelskade 11-13
7400 AC Deventer
UNIVERSITY OF BIRMINGHAM
United Kingdom
Address
Edgbaston
B15 2TT Birmingham