NEW METHODS FOR THE EVALUATION OF COMBINED EFFECTS OF ENVIRONMENTAL AGENTS ON PULMONARY MACROPHAGES AS TEST CELLS

Project Information

Grant agreement ID: EV3V0601

Project closed

Start date 1 January 1982

End date 31 December 1983

Funded under

• Sectoral research and development programme (EEC) in the field of environment (environmental protection and climatology) - indirect and concerted actions - Environmental protection -, 1981-1985

Total cost € 0,00

EU contribution No data

Coordinated by

UNIVERSITY OF COPENHAGEN
Denmark

Objective

TO ESTIMATE THE DEGREE OF TOXICITY OF SELECTED SUBSTANCE, AND COMBINATIONS THEREOF, BY CONTINUOUS OBSERVATION OF CELL METABOLISM.

MICROCALORIMETRY IS USED FOR CONTINUOUS OBSERVATION OF CHANGES IN THE METABOLIC RATE OF RABBIT PULMONARY MACROPHAGES EXPOSED TO VARIOUS COMBINATIONS OF METAL PARTICLES AND OTHER SELECTED AGENTS.

THE HEAT PRODUCTION FROM THE CELLS IS TAKEN AS A MEASURE OF THE CELL MEABOLIC LEVEL.

A COMBINATION OF SEM AND ENERGY DISPERSIVE X-RAY TECHNIQUES IS USED TO DETECT THE METAL PARTICLES CONTENT OF INDIVIDUAL CELLS, AND THE NUMBER OF PARTICLES SMALLER THAN 2 OM.

CELL VITALITY IS ESTIMATED BY DETERMINATION OF THE LACTATE DEHYDROGENAS (LD) RELEASE FROM THE CELLS.

Programme(s)

• PRE-ENVPROT 3C - Sectoral research and development programme (EEC) in the field of environment (environmental protection and climatology) - indirect and concerted actions - Environmental protection -, 1981-1985

Topic(s)

Data not available

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITY OF COPENHAGEN

• EU contribution: No data
• Address: Noerregade 10 ; 1017 KOEBENHAVN K/COPENHAEGEN ; Denmark
• Total cost: No data