NEW METHODS FOR THE EVALUATION OF COMBINED EFFECTS OF ENVIRONMENTAL AGENTS ON PULMONARY MACROPHAGES AS TEST CELLS

Informations projet

N° de convention de subvention: EV3V0601

Projet clôturé

Date de début 1 Janvier 1982

Date de fin 31 Decembre 1983

Financé au titre de

• Sectoral research and development programme (EEC) in the field of environment (environmental protection and climatology) - indirect and concerted actions - Environmental protection -, 1981-1985

Coût total € 0,00

Contribution de l’UE Aucune donnée

Coordonné par

UNIVERSITY OF COPENHAGEN
Denmark

Objectif

TO ESTIMATE THE DEGREE OF TOXICITY OF SELECTED SUBSTANCE, AND COMBINATIONS THEREOF, BY CONTINUOUS OBSERVATION OF CELL METABOLISM.

MICROCALORIMETRY IS USED FOR CONTINUOUS OBSERVATION OF CHANGES IN THE METABOLIC RATE OF RABBIT PULMONARY MACROPHAGES EXPOSED TO VARIOUS COMBINATIONS OF METAL PARTICLES AND OTHER SELECTED AGENTS.

THE HEAT PRODUCTION FROM THE CELLS IS TAKEN AS A MEASURE OF THE CELL MEABOLIC LEVEL.

A COMBINATION OF SEM AND ENERGY DISPERSIVE X-RAY TECHNIQUES IS USED TO DETECT THE METAL PARTICLES CONTENT OF INDIVIDUAL CELLS, AND THE NUMBER OF PARTICLES SMALLER THAN 2 OM.

CELL VITALITY IS ESTIMATED BY DETERMINATION OF THE LACTATE DEHYDROGENAS (LD) RELEASE FROM THE CELLS.

Programme(s)

• PRE-ENVPROT 3C - Sectoral research and development programme (EEC) in the field of environment (environmental protection and climatology) - indirect and concerted actions - Environmental protection -, 1981-1985

Thème(s)

Data not available

Appel à propositions

Data not available

Régime de financement

CSC - Cost-sharing contracts

Coordinateur

UNIVERSITY OF COPENHAGEN

• Contribution de l’UE: Aucune donnée
• Adresse: Noerregade 10 ; 1017 KOEBENHAVN K/COPENHAEGEN ; Danemark
• Coût total: Aucune donnée