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EFFECTS OF ACID WET DEPOSITION PHOTOSYNTHESIS AND TRANSPIRATION OF SPRUCE

Objective

TO GENERATE SCIENTIFIC INFORMATION IN ORDER TO ELUCIDATE THE INTERFERENCE OF AIR POLLUTION WITH PHYSIOLOGICAL PROCESSES IN FOREST TREES.

THE RESEARCH IS SPLITTED IN FOUR MAIN PHASES (YEARS) WHICH CAN OVERLAP.

PHASE 1 : CO2-EXCHANGE AND LEAF CONDUCTANCE MEASUREMENTS IN THE GREENTHOUSE (IN SITU) DURING THE GROWING SEASON. THE EFFECT OF SOIL WATER STRESS WILL BE STUDIED BY COMPARING WELL WATERED AND WATER STRESSED PLANTS IN EACH PH-GROUP, WITH RECOVERY AFTER REWATERING. RELATIVE WATER CONTENT AND WATER POTENTIAL OF THE TWIGS BE MEASURED. AT THE END OF SEASON BUFFERING CAPACITY WILL BE DETERMINED (TITRATION OF WATER EXTRACT WITH HCL AND NAOH).

PHASE 2: LABORATORY MEASUREMENTS (SIRIGOR) OF NET PHOTOSYNTHESIS, RESPIRATION AND TRANSPIRATION TO STUDY THE INTERACTION WITH ENVIRONMENTAL VARIABLES : LIGHT, TEMPERATURE AND, ESPECIALLY, LEAF TO AIR WATER VAPOUR PRESSURE DEFICIT (WATER STRESS !). DRYING CYCLES (AS IN PHASE 1) WILL BE REPEATED. DEPENDENT ON THE RESULTS, BIOCHEMICAL CHARACTERISTICS WILL BE DETERMINED. IF NEEDED (PROTEIN CONTENT, ENZYME ACTIVITIES).
AS THE END OF THE FIRST TWO SEASON CHLOROPHYL CONTENT AND CHEMICAL ELEMENT COMPOSITION (X-RAY FLUORESCENCE) WILL BE MEASURED.

PHASE 3 : DIRECTED BY THE IN PHASE 1 AND 2 OBTAINED RESULTS, STRUCTURAL RESEARCH WILL BE PLANNED, ESPECIALLY ON THE CUTICULA, CUTICULAR EROSION AND THE CONSEQUENCES FOR THE WATER BALANCE (MICROSCOPY).
EVENTUALLY, MORE BIOCHEMICAL DETERMINATIONS WILL BE DONE.

PHASE 4 : THE EFFECT OF PEAK EVENTS WILL BE STUDIED BY SPRAYING THE PLANTS ONCE OR A FEW TIMES WITH A SOLUTION OF LOW PH (<3). CO2-EXCHANGE AND LEAF CONDUCTANCE, RESPONSE AND RECOVERY RATE AND STOMATAL DYNAMICS WILL BE MEASURED IN SITU AND IN THE LABORATORY.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

UNIVERSITAIRE INSTELLING ANTWERPEN - UNIVERSITEIT ANTWERPEN
Address
Universiteitsplein 1
2610 Wilrijk (Antwerpen)
Belgium