GENETIC EFFECTS OF ENVIRONMENTAL CHEMICALS.

Project Information

Grant agreement ID: EV4V0045

Project closed

Start date 1 January 1988

End date 31 December 1991

Funded under

• Multiannual research and development programmes (EEC) in the field of the environment - Protection of the environment -, 1986-1990

Total cost No data

EU contribution No data

Coordinated by

Consiglio Nazionale delle Ricerche (CNR)
Italy

Objective

1) RELATE BIOLOGICAL CONSEQUENCES OF CHEMICAL MUTAGENS TO THE EXTENT AND SITE OF REACTION BETWEEN THE MUTAGENS AND DNA.
2) DEVELOPMENT AND VALIDATION OF METHODS TO IDENTIFY NON-GENOTOXIC CARCINOGENS.
3) DEVELOPMENT AND VALIDATION OF ASSAYS TO EVALUATE CHEMICALS CAPABLE OF INDUCING GENOMIC MUTATIONS.
4) TO DEVELOP TEST METHODS WHICH REPLACE OR REDUCE THE USE OF EXPERIMENTAL VERTEBRATE ANIMALS.
An in vitro micronucleus assay, together with immunofluorescent staining of the kinetochores by the CREST antibody, has been used in a Chinese hamster cell line (C1-1) to detect the aneuploidy inducing properties of 4 of the 5 chemicals in package A of the European Economic Community (EEC) aneuploidy programme. Colchicine, Chloral hydrate and Diazepam all gave positive results, but Econazole was only weakly positive.
1) DOSE-EFFECT RELATIONS ARE OBTAINED ON THE INDUCTION OF SCE AND CHROMOSOMAL ABERRATIONS IN BONE MARROW CELLS AND IN SPERMATAGONIA OF MICE TREATED WITH VARIOUS ETHYLATING AND METHYLATING AGENTS.

2) THE EFFECTS OF EPIGENETIC CHEMICALS ON JUNCTIONAL COMMUNICATION BETWEEN CELLS IS STUDIED.

3) IMMUNOFLUORESCENT ANTICENTROMETRIC ANTIBODIES ARE USED TO TEST THE EFFECTS OF VARIOUS COMPOUNDS IN THE INDUCTION OF MICRONUCLEI IN CHINESE HAMSTER CELL CULTURES.

4) DETERMINATION OF THE EXTENT TO WHICH HUMAN ERYTHROCYTES AND CELL- HOMOGENATES FROM PLANT MATERIAL CAN BE USED FOR THE ACTIVATION OF SOME CLASSES OF PROMUTAGENS.

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• natural sciences › biological sciences › genetics › DNA
• natural sciences › biological sciences › genetics › mutation
• natural sciences › earth and related environmental sciences › environmental sciences › pollution

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Programme(s)

• FP1-ENVPROT 4C - Multiannual research and development programmes (EEC) in the field of the environment - Protection of the environment -, 1986-1990

Topic(s)

Data not available

Call for proposal

Data not available

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Consiglio Nazionale delle Ricerche (CNR)

• EU contribution: No data
• Address: Città Universitaria Piazzale Aldo Moro 5 ; 00185 Roma ; Italy
• Total cost: No data