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Detoxification of toxic industrial waste: microbial degradation of polycarbonated biphenyls (PCBS) by sequential anaerobic-aerobic processes

Objective

To obtain information on:

1) the feasibility of a process to mineralize highly chlorinated biphenyls (formation of inorganic compounds like Coz, HCI) by a suitable sequential anaerobic-aerobic process and

2) its application (especially the factors which are determining the kinetics of the degradation process in a bio-reactor).


The proposed project consists of four parts:

1) Establishment of anaerobic microbial populations from polluted soil/sediments, capable of reductive dechlorination of highly chlorinated biphenyls to lower chlorinated biphenyls. Research is focused on the occurrence of this process in polluted soils and sediments, the kinetics of the process and the influence of various (environmental) factors, and the bacteria involved.

2) Establishment of aerobic bacterial cultures, capable of mineralizing low chlorinated biphenyls, by isolation from polluted soils/sediments or by In vivo-construction. Research is focused on the biochemistry and kinetics of degradation.

3) Genetic analysis of the isolated and in vivo-constructed aerobic PCB-degrading organisms and genetic engineering of these strains which includes i.e. the introduction of heavy metal resistance genes and marker genes. Research is focused on the molecular biology of PCB- degradation pathways and genes of heavy metal resistance.

4) Investigations on the biotechnology of the anaerobic/aerobic process. Research is focused on the procedure to clean contaminated soils and wastes in bioreactors.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Bergische Universität - Gesamthochschule Wuppertal
Address
Gaußstraße 20
42119 Wuppertal
Germany

Participants (3)

University of Kent at Canterbury
United Kingdom
Address

CT2 7NJ Canterbury
VITO - Vlaamse Instelling voor Technologisch Onderzoek NV
Belgium
Address
200,Boeretang
2400 Mol
WAGENINGEN UNIVERSITY
Netherlands
Address
4,Hesselink Van Suchtelenweg 4
6703 CT Wageningen