Objetivo Ultraviolet radiation in sunlight is thought to be responsible for the induction of skin tumors. More recently it appeared that exposure of the skin to ultraviolet B (UVB) radiation also results in systemic suppression of the immune system. The latter effect can contribute to an increase in the number of patients suffering from infectious diseases and skin cancer. Photon absorption produces reactive intermediates, e.g. triplet excited states, singlet oxygen and free radicals, and is the primary step of the interaction of UVB radiation with skin cells, followed by damage to essential biomolecules, such as DNA. Damage to cellular DNA of the skin has been implicated in photocarcinogenesis and UVB-induced immunosuppression. Our aim is to relate UVB-induced DNA damage and its repair to biological effects resulting from UVB irradiation, such as enhanced expression of relevant mRNA molecules, mutagenesis, modulation of cytokine levels as well as immunosuppression in vivo. Besides animal models, human keratinocytes will be used. The latter will be derived from sun-protected and frequently sun-exposed skin areas of young and aged individuals, as well as from actinic keratoses and squamous cell carcinomas. The types of DNA damage and their relative amounts will be measured with biochemical, physico-chemical, and immunochemical techniques. The effect of anti-oxidants on the occurrence of certain types of UVB-induced DNA damage and on the related biological effects will also be assessed. From this information the contribution of certain types of UVB-induced cellular damage to skin cancer and immunosuppression may be resolved. This knowledge will provide a scientific basis for protective or prophylactic measures that may be effective in cancer prevention. Ámbito científico ciencias naturalesciencias biológicasgenéticaADNciencias naturalesciencias biológicasbioquímicabiomoléculasciencias médicas y de la saludciencias de la saludenfermedad infecciosaciencias médicas y de la saludmedicina clínicaoncologíamelanomacarcinoma de células escamosasciencias médicas y de la saludmedicina básicainmunología Programa(s) FP3-ENV 1C - Specific research and technological development programme (EEC) in the field of the environment, 1990-1994 Tema(s) 040203 - Environment and human health Convocatoria de propuestas Data not available Régimen de financiación CSC - Cost-sharing contracts Coordinador NETHERLANDS ORGANISATION FOR APPLIED SCIENTIFIC RESEARCH - TNO Dirección 48,utrechtseweg 48 3700 AJ Zeist Países Bajos Ver en el mapa Aportación de la UE Sin datos Participantes (3) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo Bayerische Julius-Maximilians-Universität Würzburg Alemania Aportación de la UE € 0,00 Dirección Versbacher straße 97078 Würzburg Ver en el mapa Otras fuentes de financiación Sin datos Katholieke Universiteit Leuven Bélgica Aportación de la UE € 0,00 Dirección 49,herestraat 3000 Leuven Ver en el mapa Otras fuentes de financiación Sin datos LEIDEN UNIVERSITY Países Bajos Aportación de la UE € 0,00 Dirección 55,einsteinweg 55 gorlaeus laboratories 2333 CC Leiden Ver en el mapa Otras fuentes de financiación Sin datos
