Objective
The specific objectives of this research project are: The identification Of markers differentially expressed in juvenile versus mature phases of tree species which include: the generation and characterization of phase specific CDNA markers by means of differentially expressed genes. the analysis of anti-oxydant isozyme patterns related to phase change the analyses of changes in meristem profile and zonation during aging. the characterization of developmental stages by in vivo monitoring of cytoplasmic pH, changes in membrane potential and ion distribution. the determination of specific plant growth regulator ratios and signaling to characterize maturation and rejuvenation.
The testing of such markers for specificity across a range of species (Gymnosperms and Angiosperms) and tissues (Juvenile and mature) to determine their general applicability.
The performance of field trials at larger scale for validation.
During this project different types of markers for phase change from juvenile to mature stage will be identified in certain model species (peach, apple, pines,...) and developed for different forest species (Pinus sp., Prunus avium). These markers include molecular markers based on tissue specific mRNAs, isozymes (CAT, SOD), plant growth regulator ratios (Auxins, Cytokinins, ABA, Ethylene,PAs, Phenols, Ion content) and other physiological, histological and morphological markers. In order to test their specificity and general applicability, molecular markers developed by individual participants will be interchanged and tested by other groups on different plant species and using different juvenile, mature and rejuvenated tissues (e.g. shoots, nodes, buds, phytomers; seedlings and young progeny genotypes, cascade graftings, cuttings, micrograftings, micropropagated material). On the other hand, plant material of different species will be made available to the participants studying isozymes, plant growth regulators, physiological and histo-morphological markers in order to extent their analyses to other species and tissues. Plant material with known favourable and unfavourable propagation behaviour will be analyzed with previously established markers. Propagation and flowering behaviour, as predicted by marker analyses, will be verified at larger commercial scale through field trials.
Phase specific markers would offer the possibility to identify genotypes with prolonged Juvenile phase, to evaluate any process for rejuvenation, or to identify genotypes with early flowering for seed orchards. In this way powerfools tools to manipulate propagation (afforestation) and for tree selection and breeding would be available.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
01080 VITORIA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.