Objective
The objectives of the proposed project are:
(i) to develop where necessary, optimise and evaluate the use of DNA profiling methods (microsatellites and AFLP) for plant variety identification and registration and for hybrid purity assessment in a number of crops of interest to European agriculture (maize, sunflower, and Brassica crops, primarily oilseed rape and the vegetable white kale).
(ii) to use maize as a model species for examining hybrids of various types and for comparison with the recognised and widely used biochemical methods for hybrid purity assessments.
(iii) to use oilseed rape and kale as models for other Brassica crops where hybrid varieties of different types are commonly produced (e.g. Brussels sprouts, cabbage, cauliflower), to compare the results obtained with those from existing biochemical/molecular methods and to examine the transportability of techniques between species within the genus.
(iv) to develop not only the necessary profiling technology, but also the overall methodology required for the use of the techniques in the seed testing and certification context and to devise an operational system. This would involve consideration of both technical and statistical issues.
(v) to organise a series of 'ring tests' which would compare the data obtained from the use of the optimised method(s) with a particular seed lot with those obtained from existing biochemical analysis, where appropriate, and observed in field plots of the same lot. Whilst this comparison would apply primarily to the overall level of hybrid purity determined by the different approaches, it would also include the examination of any 'off-types'. This would form part of the assessment of an optimised operational system for seed certification.
(vi) to develop databases of varietal DNA profiles and begin to examine the potential applications of such techniques and databases to varietal registration.
Hence overall the proposed research will promote collaborative research and the development of harmonised and efficient systems for plant variety testing throughout the EU, aiming to ensure the availability of high quality varieties to the agricultural industry and consumers. It will provide tools for production of higher quality raw materials which in turn will increase the efficiency of the agri-industries within Europe. In addition, the DNA profiling systems can be used readily to trace varieties (and the products derived from them) throughout 'total quality management' systems, from propagative material to the end product, both within and between companies. This will also contribute to the improvement of the quality of produce.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences computer and information sciences databases
- natural sciences biological sciences genetics DNA
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds oilseeds
- agricultural sciences agriculture, forestry, and fisheries agriculture horticulture vegetable growing
- agricultural sciences agriculture, forestry, and fisheries agriculture grains and oilseeds cereals
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
CB3 0LE Cambridge
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.