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THE ASSESSMENT OF GENETIC PURITY IN HYBRID VARIETIES OF CROPS

Objectif



The objectives of the proposed project are:
(i) to develop where necessary, optimise and evaluate the use of DNA profiling methods (microsatellites and AFLP) for plant variety identification and registration and for hybrid purity assessment in a number of crops of interest to European agriculture (maize, sunflower, and Brassica crops, primarily oilseed rape and the vegetable white kale).
(ii) to use maize as a model species for examining hybrids of various types and for comparison with the recognised and widely used biochemical methods for hybrid purity assessments.
(iii) to use oilseed rape and kale as models for other Brassica crops where hybrid varieties of different types are commonly produced (e.g. Brussels sprouts, cabbage, cauliflower), to compare the results obtained with those from existing biochemical/molecular methods and to examine the transportability of techniques between species within the genus.
(iv) to develop not only the necessary profiling technology, but also the overall methodology required for the use of the techniques in the seed testing and certification context and to devise an operational system. This would involve consideration of both technical and statistical issues.
(v) to organise a series of 'ring tests' which would compare the data obtained from the use of the optimised method(s) with a particular seed lot with those obtained from existing biochemical analysis, where appropriate, and observed in field plots of the same lot. Whilst this comparison would apply primarily to the overall level of hybrid purity determined by the different approaches, it would also include the examination of any 'off-types'. This would form part of the assessment of an optimised operational system for seed certification.
(vi) to develop databases of varietal DNA profiles and begin to examine the potential applications of such techniques and databases to varietal registration.
Hence overall the proposed research will promote collaborative research and the development of harmonised and efficient systems for plant variety testing throughout the EU, aiming to ensure the availability of high quality varieties to the agricultural industry and consumers. It will provide tools for production of higher quality raw materials which in turn will increase the efficiency of the agri-industries within Europe. In addition, the DNA profiling systems can be used readily to trace varieties (and the products derived from them) throughout 'total quality management' systems, from propagative material to the end product, both within and between companies. This will also contribute to the improvement of the quality of produce.

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Coordinateur

National Institute of Agricultural Botany (NIAB)
Contribution de l’UE
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Adresse
Huntingdon Road
CB3 0LE Cambridge
Royaume-Uni

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