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Diagnosis of oyster herpes-like virus: Development and validation of molecular, immunological and cellular tools.

Objective

The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in oysters and to validate these reagents by using them in different European laboratories involved in bivalve epidemiological surveys.

This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are :

1. Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure.
2. Comparing oyster herpes-like viruses with other viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure.
3. Developing molecular tools for oyster herpes-like virus detection using the complete virus DNA sequence data.
4. Developing immunological tools for oyster herpes-like virus detection.
5. Developing cellular tools for oyster herpes-like virus detection using oyster primary cell cultures and vertebrate cell lines.
6. Application of developed diagnostic tools for herpes-like virus detection in oyster samples from different geographical locations.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

IFREMER - DRV/RA
Address
Mus De Loup
17390 La Tremblade
France

Participants (6)

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
Address
Calle Serrano 117
Madrid
MEDICAL RESEARCH COUNCIL
United Kingdom
Address
Church Street
G11 5JR Glasgow
Pharos SA
Belgium
Address
14,Rue Bois Saint-jean
4102 Seraing
The Centre for Environment, Fisheries and Aquaculture Science
United Kingdom
Address
Barrack Road, The Nothe
DT4 8UB Weymouth, Dorset
Universite de Bretagne Occidentale
France
Address
Avenue Le Gorgeu N° 6
29285 Brest
University College Cork
Ireland
Address
Lee Maltings, Prospect Row
Cork