Diagnosis of oyster herpes-like virus: Development and validation of molecular, immunological and cellular tools.

Objective

The aim of the programme is to develop specific tools for diagnosing herpes-like virus infections in oysters and to validate these reagents by using them in different European laboratories involved in bivalve epidemiological surveys.

This will done using techniques developed by the partners to characterise viruses by studying their genomes and immunologically reactive proteins, to cultivate oyster cells and vertebrate cell lines and to perform epidemiological surveys among bivalves. The programme objectives are :

1. Obtaining the complete oyster herpes-like virus DNA sequence with determination of the genome structure.
2. Comparing oyster herpes-like viruses with other viruses belonging to the Herpesviridae family on the basis of sequence data and genome structure.
3. Developing molecular tools for oyster herpes-like virus detection using the complete virus DNA sequence data.
4. Developing immunological tools for oyster herpes-like virus detection.
5. Developing cellular tools for oyster herpes-like virus detection using oyster primary cell cultures and vertebrate cell lines.
6. Application of developed diagnostic tools for herpes-like virus detection in oyster samples from different geographical locations.

Programme(s)

• FP4-FAIR - Specific research, technological development and demonstration programme in the field of agriculture and fisheries (including agro-industry, food technologies, forestry, aquaculture and rural development), 1994-1998

Topic(s)

• 5.3.2 - Health of aquacultured species

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

IFREMER - DRV/RA

Address

Mus De Loup
17390 La Tremblade
France

Participants (6)

CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS

Spain

Address

Calle Serrano 117
Madrid

MEDICAL RESEARCH COUNCIL

United Kingdom

Address

Church Street
G11 5JR Glasgow

Pharos SA

Belgium

Address

14,Rue Bois Saint-jean
4102 Seraing

The Centre for Environment, Fisheries and Aquaculture Science

United Kingdom

Address

Barrack Road, The Nothe
DT4 8UB Weymouth, Dorset

Universite de Bretagne Occidentale

France

Address

Avenue Le Gorgeu N° 6
29285 Brest

University College Cork

Ireland

Address

Lee Maltings, Prospect Row
Cork