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Contenido archivado el 2024-06-10

Functional analysis of acta, a bacterial protein involved in actin-based mobility

Objetivo



Listeria monocytogenes is an intracellular human pathogen. It uses actin and other actin- associated proteins to move through the host cell cytoplasm. The surface protein ActA is the sole listerial factor needed for recruitment of host cell actin. It is polarly distributed on the bacterial surface. To identify the structural features of ActA crucial for activity and its polarized distribution I plan to: - perform deletions and point mutation analysis of different domains of ActA, followed by the analysis of the mutants in infected cells or in cytoplasmic extracts. - analyse if there is a specific sequence in ActA which serves as a targeting signal for its polar distribution.
The results of this project will help us to understand actin-based bacterial motility, a phenomenon which plays a crucial role in the dissemination of Listeria monocytogenes in the liver.

Ámbito científico (EuroSciVoc)

Programa(s)

Tema(s)

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

RGI - Research grants (individual fellowships)

Coordinador

INSTITUT PASTEUR
Aportación de la UE
Sin datos
Dirección
25,Rue du Docteur Roux 28
75724 PARIS
Francia
Coste total
Sin datos

Participantes (1)