Physiology of recombinant glucose-derepressed saccharomyces cerevisae strain

Objective

Glucose repression in yeast represents a major problem for both the cultivation on industrial substrates and for the breadmaking (leavening) because glucose has to be depleted before the remaining sugars can be utilized. In glucose-derepressed Saccharomyces cerevisiae strains, simultanous, and concomitantly more time-efficient, utilisation of the sugars would be possible resulting in an improved process economy and a lower environmental burden beside better leavening characteristics. Several genes playing key roles in catabolite repression have been deleted in an industrial strain. The physiology of the recombinants will be compared with the wild type in well controlled high performance bioreactors in order to elucidate the respective gene functions in the regulatory cascade of catabolite repression. Measurements of extracellular sugars and metabolites, assays for sugar-cleavaging enzymes and uptake systems, Northern blotting and radioactive techniques will be the analytical tools providing the basis for a metabolic flux analysis.

Fields of science

• engineering and technology › environmental biotechnology › bioremediation › bioreactors
• medical and health sciences › basic medicine › physiology
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL16 - Biotechnology

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

TECHNICAL UNIVERSITY OF DENMARK

Address

Dtu, Blgd. 221
2800 Lyngby
Denmark

Participants (1)

Not available

Germany