Objective
The project proposed constitutes an extension of that carried out during my stay in the University of Padova. I will first construct a set up to measure aequorin luminescence, similar to those actually running in Padova. Then, I will use the HeLa clones I have obtained in Padova, which stably express the DNA construct designed to target aequorin (both native and mutated with low Ca2+ affinity) to the endoplasmic reticulum (ER). These cells will be used to continue the study of ER-Ca2+-heterogeneity currently being carried out in Padova. At the same time, I plan to produce a series of point mutations in the Ca2+ -binding sites of aequorin in order to reduce its Ca2+-affinity up to near the mM range. In the mean time, Sr2+ will be used as a low-affinity Ca2+-surrogate. Sr2+ has been shown to behave similarly to Ca2+ in terms of uptake, accumulation and release from the ER.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences biological sciences genetics mutation
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
47005 Valladolid
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.