Objective
The aim of this work is to search for genes involved in the regulation global responses in Lactococcus lactis. To achieve our objective, we propose to make several transcriptional fusions of promoters from cloned L. lactis genes to promoterless reporter genes (cat, lacLM, lacZ, gus, lacG) and inserted them in the lactococcal chromosome. One derivative with more than one fusion will be used to obtain a collection of insertional mutants by using a Ts-system based on an IS element (ISS1) developed in the host institution. Mutants with higher resistance to Cm or with less intense blue colour will be selected and the mutated gene will be transferred to the strains containing the other fusions in order to analyze its ability to regulate them. Further characterization of pleiotropic regulators will be made on the molecular level regarding the structure and expression of these regulatory genes and its targets.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology materials engineering colors
- natural sciences biological sciences genetics chromosomes
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
78352 Jouy-en-Josas
France
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