Skip to main content
Aller à la page d’accueil de la Commission européenne (s’ouvre dans une nouvelle fenêtre)
français fr
CORDIS - Résultats de la recherche de l’UE
CORDIS
Contenu archivé le 2024-05-07

Research for general regulators in lactococcus lactis

Objectif



The aim of this work is to search for genes involved in the regulation global responses in Lactococcus lactis. To achieve our objective, we propose to make several transcriptional fusions of promoters from cloned L. lactis genes to promoterless reporter genes (cat, lacLM, lacZ, gus, lacG) and inserted them in the lactococcal chromosome. One derivative with more than one fusion will be used to obtain a collection of insertional mutants by using a Ts-system based on an IS element (ISS1) developed in the host institution. Mutants with higher resistance to Cm or with less intense blue colour will be selected and the mutated gene will be transferred to the strains containing the other fusions in order to analyze its ability to regulate them. Further characterization of pleiotropic regulators will be made on the molecular level regarding the structure and expression of these regulatory genes and its targets.

Champ scientifique (EuroSciVoc)

Programme(s)

Thème(s)

Appel à propositions

Données non disponibles

Régime de financement

RGI - Research grants (individual fellowships)

Coordinateur

Institut National de la Recherche Agronomique
Contribution de l’UE
Aucune donnée
Adresse
Domaine de Vilvert
78352 Jouy-en-Josas
France
Coût total
Aucune donnée

Participants (1)