Objective
The human U1 small nuclear ribonucleoprotein (snRNP) is one of a family snRNPs that play an essential role in the removal of introns from pre-messenger RNA (pre-mRNA). The U1 snRNP-specific U1A protein autoregulates its production by binding to its own pre-mRNA and inhibiting polyadenylation. Two molecules of U1A were shown to bind to a conserved region in the 3' untranslated region of U1A pre-mRNA to produce efficient inhibition of polyadenylation. The mechanism of this regulation has been elucidated in vitro: U1A protein bound to its own pre-mRNA inhibits polyadenylation activity of poly(A) polymerase (PAP) by a direct and specific interaction between U1A and PAP.
The tripartite complex between U1A pre-mRNA, U1A protein and PAP and th relationship between its structure and function in inhibition of polyadenylation i71 vitro will be investigated. I will then experiment the possibility that the U1APAP interaction used in autoregulation of U1A protein synthesis might also be involved in either the other example of U1A regulation of polyadenylation (of SV40 late mRNA) or in the coupling of splicing and polyadenylation that has been shown to exist.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
HEIDELBERG
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.