Objectif The human U1 small nuclear ribonucleoprotein (snRNP) is one of a family snRNPs that play an essential role in the removal of introns from pre-messenger RNA (pre-mRNA). The U1 snRNP-specific U1A protein autoregulates its production by binding to its own pre-mRNA and inhibiting polyadenylation. Two molecules of U1A were shown to bind to a conserved region in the 3' untranslated region of U1A pre-mRNA to produce efficient inhibition of polyadenylation. The mechanism of this regulation has been elucidated in vitro: U1A protein bound to its own pre-mRNA inhibits polyadenylation activity of poly(A) polymerase (PAP) by a direct and specific interaction between U1A and PAP. The tripartite complex between U1A pre-mRNA, U1A protein and PAP and th relationship between its structure and function in inhibition of polyadenylation i71 vitro will be investigated. I will then experiment the possibility that the U1APAP interaction used in autoregulation of U1A protein synthesis might also be involved in either the other example of U1A regulation of polyadenylation (of SV40 late mRNA) or in the coupling of splicing and polyadenylation that has been shown to exist. Champ scientifique sciences naturellessciences biologiquesbiochimiebiomoléculeprotéinessciences naturellessciences biologiquesgénétiqueARN Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Thème(s) 0302 - Post-doctoral research training grants TL02 - Molecular Biology and Biochemistry Appel à propositions Data not available Régime de financement RGI - Research grants (individual fellowships) Coordinateur EUROPEAN MOLECULAR BIOLOGY LABORATORY Adresse Meyerhofstrasse 1 Heidelberg Allemagne Voir sur la carte Contribution de l’UE € 0,00 Participants (1) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire Not available France Contribution de l’UE € 0,00 Adresse Voir sur la carte
