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Characterization and isolation of the blue light photoreceptor of the no3-, no2- and c1- uptake system(s) in monoraphidium braunii

Objectif



Plasma membrane vesicles will be isolated and used to study the mechanism(s) that controls monovalent anion uptake in the green alga Monoraphidium braunii. These anions (NO3-, NO2- and Cl-) are essential for the development of all plant cells. The activation of this uptake system depends on the presence of blue/UV radiation in the actinic light. Using plasma membrane vesicles, we will attempt to localize, identify and, if possible, purify the photoreceptor(s) involved. Recent experiments suggest that in vivo a cyclic GMP-dependent phosphorylation occurs during the activation of the uptake mechanism. The role of protein phosphorylation in the sensory transduction of the blue/UV light-dependent activation of these transport processes will also be studied. Conversely, a photosynthetic rate approaching saturation is required for the full activation of the entrance of monovalent anions in the cells. The requirements into energy and/or reducing power for the transport of these anions will also be investigated using plasma membrane vesicles.

Champ scientifique (EuroSciVoc)

Programme(s)

Thème(s)

Appel à propositions

Données non disponibles

Régime de financement

RGI - Research grants (individual fellowships)

Coordinateur

Lund University
Contribution de l’UE
Aucune donnée
Adresse
35,Sölvegatan
221 00 Lund
Suède
Coût total
Aucune donnée

Participants (1)