Role of (a/v)tlxt(t/s) sequence in the regulation of l-amino acid decarboxylases

Project Information

Grant agreement ID: FMBI960874

Project closed

Start date 1 July 1996

End date 30 June 1997

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost € 0,00

EU contribution No data

Coordinated by

Universidad de Málaga
Spain

Objective

Polyamines play an essential role in cell growth and differentiation, and their biosynthesis pathway may be an important target for design of chemotherapeutic agents. Ornithine decarboxylase (ODC) is the first and rate-limiting enzyme in polyamine biosynthesis. ODC is a highly regulated enzyme with contro] points at the level of transcription, translation and protein degradation. Indeed, ectopic expression of ODC conferred a transformed phenotype to mouse fibroblast. These results suggest that modulation of ODC activity is important for growth regulation. Recently a consensus sequence, (AIV)TLXT(T/S), has been identified which is present in all mammalian L-amino acid decarboxylases that are regulated by polyamines, has been detected. The objective of the proposed project is to characterize the role of this sequence in the regulation of ODC expression and activity. Transfection of cells with different ODC construct carrying specific mutations of this sequence will be use. The results of these studies will be extended to other selected L-amino acid decarboxylases.

Fields of science

• natural sciences › biological sciences › genetics › mutation
• natural sciences › biological sciences › biochemistry › biomolecules › proteins › enzymes

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL05 - Cell Biology

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

Universidad de Málaga

• EU contribution: No data
• Address: S/N,Campus de Teatinos ; 29071 Málaga ; Spain
• Total cost: No data

Participants (1)

Not available

Spain

• EU contribution: No data
• Total cost: No data