Objective
The objective of this work is to develop strategies for controlling, in continuous mammalian cell lines, the expression of human-type glycoproteins used as biopharmaceuticals. Investigation will focus on parameters affecting the final oligosaccharide structure and hence the biological properties of recombinant human glycoproteins. The study is based on the novel hypothesis that the intracellular pool of UDP-N-acetylhexosamines (UDP-HexNAc) affects the expression of Nglycans. This pool is modulated by the culture environment, as both glutamine (via ammonium ion release) and glucose can contribute to it. Specific aims of the proposed project include: (a) Characterization of the effects of chemical parameters affecting UDP-HexNAc pool on the carbohydrate expression of a model glycoprotein produced by recombinant mammalian cells. (b) Evaluation of the effect of a shift in intracellular pH or culture temperature on carbohydrate expression. (c) Elucidation of the mechanisms responsible for changes in carbohydrate expression patterns resulting from changes in physical and chemical parameters.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
54006 Thessaloniki
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.