In vitro model of lympho-hematopoietic differentiation from murine embryonicstem(es) cell. Characterization of lineage specific gene expression and cytokines requirements

Objective

The development of T and B lymphocytes characterized by a precise sequence of maturatlon steps, some of which defined by the expression of specific cell surface markers.
Aim of this research project is to develop a system for the analysis of the molecular and cellular aspects during the early phases of lymphoid development : the first effort will be devoted to the establishment of reproducible system for generating T and/or B lymphocytes from murine embryonic stem cells (ES) in vitro. A combination of varius cytokines and several stromal cell lines known to have a potent lymphohematopoietic promoting activity will be used on these undifferentiated, pluripotent cells, both to elucidate the relative role of these cytokines in lymphoid development and to better understand T cell differentiation Secondly, this system will be used to identify new genes involved in the lymphoid differentiation pathways, using a strategy based on the gene-trap approach.

Fields of science

• medical and health sciences › medical biotechnology › cells technologies › stem cells

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL07 - Immunology and Cancer

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

Address

149,rue De Sèvres 149 - Hopital Necker Enfants Malades
75015 Paris
France

Participants (1)

Not available

Italy