Objective The development of T and B lymphocytes characterized by a precise sequence of maturatlon steps, some of which defined by the expression of specific cell surface markers. Aim of this research project is to develop a system for the analysis of the molecular and cellular aspects during the early phases of lymphoid development : the first effort will be devoted to the establishment of reproducible system for generating T and/or B lymphocytes from murine embryonic stem cells (ES) in vitro. A combination of varius cytokines and several stromal cell lines known to have a potent lymphohematopoietic promoting activity will be used on these undifferentiated, pluripotent cells, both to elucidate the relative role of these cytokines in lymphoid development and to better understand T cell differentiation Secondly, this system will be used to identify new genes involved in the lymphoid differentiation pathways, using a strategy based on the gene-trap approach. Fields of science medical and health sciencesmedical biotechnologycells technologiesstem cells Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL07 - Immunology and Cancer Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE Address 149,rue de sèvres 149 - hopital necker enfants malades 75015 Paris France See on map EU contribution € 0,00 Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Italy EU contribution € 0,00 Address See on map
