In vitro model of lympho-hematopoietic differentiation from murine embryonicstem(es) cell. Characterization of lineage specific gene expression and cytokines requirements

Project Information

Grant agreement ID: FMBI961014

Project closed

Start date 1 September 1996

End date 31 August 1998

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
France

Objective

The development of T and B lymphocytes characterized by a precise sequence of maturatlon steps, some of which defined by the expression of specific cell surface markers.
Aim of this research project is to develop a system for the analysis of the molecular and cellular aspects during the early phases of lymphoid development : the first effort will be devoted to the establishment of reproducible system for generating T and/or B lymphocytes from murine embryonic stem cells (ES) in vitro. A combination of varius cytokines and several stromal cell lines known to have a potent lymphohematopoietic promoting activity will be used on these undifferentiated, pluripotent cells, both to elucidate the relative role of these cytokines in lymphoid development and to better understand T cell differentiation Secondly, this system will be used to identify new genes involved in the lymphoid differentiation pathways, using a strategy based on the gene-trap approach.

Fields of science

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques.

• medical and health sciences › medical biotechnology › cells technologies › stem cells

Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL07 - Immunology and Cancer

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

• EU contribution: No data
• Address: 149,Rue de Sèvres 149 - Hopital Necker Enfants Malades ; 75015 PARIS ; France
• Total cost: No data

Participants (1)

Not available

Italy

• EU contribution: No data
• Total cost: No data