Objective I am interested in how growth factors regulate uni-lineage commitment and differentiation programmes in haematopoiesis i.e. do they (i) induce rate limiting changes and therefore specify lineage directly or (ii) simply promote the proliferation and/or maturation of cells that have already made commitment decisions in an independent manner. We will focus on the macrophage colony stimulating factor receptor (M-CSF-R) asking (i) can enforced M-CSF-R expression in multipotential cells instruct macrophage lineage specification and (ii) can the expression of tne MCSF-R in erythroid-committed cells block, or reverse, the erythroid-lineage specification programme and support a transdifferentiation of these cells into macrophages. In order to target the expression of human M-CSF-R to the desired haematopoietic compartments we will take a "knock-in" approach through homologous recombination in murine embryonic stem (ES) cells; we will target into the CD341stem cell antigen locus to obtain expression in multipotential cells and into the erythropoietin receptor locus to obtain expression in erythroid-committed cells. Programme(s) FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998 Topic(s) 0302 - Post-doctoral research training grants TL02 - Molecular Biology and Biochemistry Call for proposal Data not available Funding Scheme RGI - Research grants (individual fellowships) Coordinator INSTITUTE OF CANCER RESEARCH : ROYAL CANCER HOSPITAL Address 237,fulham road 237 SW3 6JB London United Kingdom See on map EU contribution No data Participants (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all Not available Italy EU contribution € 0,00 Address See on map Other funding No data
