Objective
A huge variety of different intracellular signalling events initiated by either the extracellular environment or intracellular conditions is operative in intact living cells. For the sake of simplicity, these pathways are usually considered as almost separate entities. Some light has recently been shed upon a cross point of two possibly interconnected pathways. One is initiated by activated trimeric GTP-binding proteins and the other through receptors that have intrinsic tyrosine kinase activity upon stimulation. The cross point is thought to be the tyrosine kinase PYK2. I am going to investigate this kinase and its partners in a model system where I shall take advantage of the diversity of recently characterized different PC12 clones. These PC12 clones are quite diverse in terms of expression of a variety of marker proteins as well as in behaviour towards extracellular stimuli, thus providing an interesting tool to dissect the intracellular signalling pathways acting in these clones. Activity determination of various kinases, phosphatases, transcription factors and genes will be established and in a further step all these activities will be compromised and the reactions of the so treated clones towards extracellular stimuli will be investigated. With this approach the putative links between the two signalling pathways should become more clearer.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences cell biology cell signaling
- natural sciences biological sciences biochemistry biomolecules proteins
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
20132 MILANO
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.