Objective
Research objectives and content
The objective of this proposal is to produce large quantities of small oligopeptides in a strain(s) of the lactic acid bacterium, Lactococcus lactis. A particular strain of L. Iactis, shown under certain conditions accumulate intact oligopeptides intracellularly, is available in the host institute. This strain will be further manipulated to remove two additional endopeptidase activities. The strain(s) will subsequently be used to express synthetic genes encoding for the oligopeptides met-enkephalin, bradykinin, and a-casein fragment f(1-23). Genetic and physiological parameters will be examined to optimize levels of gene expression and oligopeptide production and purification. Training content (objective, benefit and expected impact)
The training content of this project would offer an expert (the applicant) in biochemical techniques involving protein/peptide purification and characterization the opportunity to obtain expertise in molecular biology. Therefore, this project would allow for the integration of expertise of both the applicant and the host institute. Links with industry / industrial relevance (22)
The proposal is of pre-competitive nature. It will allow the possibilities of producing oligopeptides directly in bacteria to be ascertained. Successful production of oligopeptides using a specific strain developed in this proposal could be of significant industrial relevance. This project could allow the development of an innovative and economically viable alternative to existing methods of peptide synthesis. The proposed method has the potential to efficiently and accurately produce oligopeptides in large quantities and may be superior to existing chemical synthesis or gene fusion methods.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry organic acids
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
9747 AG GRONINGEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.