Objective
Research objectives and content
This project is designed to identify novel mouse genes expressed in a segment-specific manner in developing hindbrain, without any presupposition on their identity. We will take advantage of the existence of mice carrying a null mutation in the gene Krox-20, which results in an elimination of rhombomeres number 3 and 5. We will apply the procedure of Differential RNA Display to this very clean genetic system, which should allow the identification of genes specifically expressed in the segments affected by the mutation. In practice, we have already performed preliminary experiments which have led to the cloning of several sequences which are amplified with wild type RNA but not with mutant RNA. We will continue this analysis to accumulate additional candidate clones. These will then be used in whole mount in situ hybridisation experiments to establish whether their expression is indeed restricted to rhombomeres 3 or 5 during hindbrain development. The positive clones will be sequenced and, if they correspond to novel genes, they will be used to isolate complete cDNAs in order to initiate their functional analysis. Particular efforts will be put on genes encoding adhesion molecules, if such genes are revelad by this work.
Training content (objective, benefit and expected impact)
The morphogenesis of the vertebrate hindbrain is subjected to a segmentation process which sets essential aspects of its neuronal architecture. To have access to the molecular mechanisms governing this phenomenon, it is essential to identify the genes which play a role in its control. It is expected that many of these will be expressed in a segment-specific manner. Indeed a few genes with such an expression pattern have been identified, either by accident or by systematic search among known gene families. In this project, we plan to identify novel genes expressed specifically in segments 3 or 5 and to initiate their functional analysis. If successful, this work should open the door to major extension of our knowledge of the genetic control of hindbrain segmentation.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics mutation
- natural sciences mathematics pure mathematics mathematical analysis functional analysis
- natural sciences biological sciences genetics RNA
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75230 PARIS
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.