Objective
Research objectives and content The alm of the research is to elucidate the nature of dynamic protein:protein interactions. Why are some complexes of proteins extremely static while others, in particular complexes of redox proteins, have very high turnover rates ? What are the roles in complex formation of the various non-covalent forces, such as electrostatic and hydrophobic forces and hydrogen bonds ? The project will analyze the structural and dynamic properties of a dynamic protein:protein complex in detail by a combination of kinetic measurements, site-directed mutagenesis and a new NMR approach. The latter is based on analysis of chemical shift changes caused by a) binding effects and b) intermolecular paramagnetic effects. This method is an excellent tool to obtain detailed information on the interaction sites of the two proteins and it has made it possible to determine the structure the complex of plastocyanin ( 10 kDa) and cytochrome (28 kDa), two redox proteins that have a crucial function in photosynthesis. In the project this new method will be exploited to study the effects on complex formation of the various non-covalent forces separately. Mutagenesis will be used to manipulate properties such as charge distribution and surface shape and polarity and the complexes of these mutants proteins will analyzed with NMR and by kinetic measurements. Furthermore, NMR will be used to study the dynamic properties of the proteins in the complex. Training content (objective, benefit and expected impact) Training objectives are twofold. First, to become fully competent as an experimental NMR spectroscopist, able to use multidimensional techniques for the study of proteins, and, second, to get familiar with the latest-developments in methods for site-directed mutagenesis and protein purification.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences botany
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
54006 Thessaloniki
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.