Regulation of mammalian iron metabolism by oxidative stress

Información del proyecto

Identificador del acuerdo de subvención: FMBI971978

Fecha de inicio

Fecha de finalización

Financiado con arreglo a

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Coste total

Sin datos

Aportación de la UE

Sin datos

Coordinado por

Aristotle University of Thessaloniki
Greece

Objetivo

In the proposed research plan, we intend to study physiological and mechanistic aspects of the response of IRP-1 to oxidative stress. By employing different agents which cause oxidative stress to cells, we will compare the effects of extracellular and intracellular oxidative stress on IRP-1. For this, we need to measure extra- and intracellular H2o2 levels. A highly sensitive chemiluminescent assay will be applied for the determination of H2o2 in the tissue culture supernatant (Mueller and Arnhold, 1995), while intracellular levels of H2o2 will be assessed by FACS analysis, using a fluorescent redox- sensitive probe. These tools will enable a detailed characterisation of the effects of H2r2 on IRP-1. In our previous work, we have provided evidence that extracellular H2o2 causes an accelerated turnover of the 4Fe-4S cluster, possibly via a signaling pathway. We now propose to further address this hypothesis, aiming to elucidate the underlying mechanism. This would require to establish an in vitro system to recapitulate the effects observed in intact cells. Such a system would enable us to dissect the early steps of the H202-response from the actual removal of the 4Fe-4S cluster. Furthermore it would provide a framework to isolate and biochemically characterize possible cluster- destabilizing activities. Training, content (objective, benefit and expected impact) The goals of this project lie within the interests of the host lab. The choise of this host lab is appropriate, as my previous training at EMBL on modern techniques of molecular biology will be meaningfully complemented by the expertise and know-how of Dr. Papavassiliou's lab. In addition, I believe that I could contribute with my knowledge in the undergraduate as well as graduate programmes of the Medical School of Patras.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias biológicas › biología molecular

Programa(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Tema(s)

• 0303 - Return grants

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

Datos no disponibles

Coordinador

Aristotle University of Thessaloniki

• Aportación de la UE: Sin datos
• Dirección: Analytical Chemistry Lab ; 54006 Thessaloniki ; Grecia
• Coste total: Sin datos