Skip to main content
Aller à la page d’accueil de la Commission européenne (s’ouvre dans une nouvelle fenêtre)
français français
CORDIS - Résultats de la recherche de l’UE
CORDIS
Contenu archivé le 2024-04-30

A-l-fucosidase and its use to modify the xyloglucan-cellulose network of plant cell walls

Objectif



Research objectives and content
The texture (and hence quality) of vegetables and fruits is influenced strongly by the mechanical properties of the plant cell wall. Although there is considerable knowledge of the structures of cell wall polymers and of the mechanical properties of cell walls and tissues, a major and persistent problem is a lack of knowledge of how altered molecular structures within cell walls translate into changes in mechanical properties.
My doctoral work in Spain has focused on the role of the cell wall associated peroxidases in the stiffening of the cell wall during growth. It would greatly enhance my future research potential if I were to gain experience in the major polysaccharide networks of cell walls, and at the same time acquire experience in molecular biology. I would therefore like to join of Professor J S Grant Reid's laboratory to participate in an investigation of the effect of xyloglucan structure modification on the properties of xyloglucan-cellulose networks.
The objectives of the work I propose in Professor Reid's laboratory are (1) to purify a xyloglucan-active a-L-fucosidase from germinated nasturtium seeds in quantities sufficient to allow its use to modify xyloglucan structure in vitro on a hundreds-of milligrams scale (2) to prepare fucose-modified xyloglucans to be assessed, in collaboration with Industry, in novel cell wall like in vitro-assembled cellulose-xyloglucan networks and (3) to obtain a cDNA clone of the fucosidase with a view to future overexpression of the enzyme.
Training content (objective, benefit and expected impact)
Professor Reid's laboratory at U Stirling enjoys world status in the isolation and molecular characterization of natural plan enzymes capable of modifying the important cellulose-xyloglucan network of the plant cell wall. By joining this active group shall learn plant enzymology and molecular biology in the context described above. and also gain exposure to their highly specialized systems to study plant cell wall polysaccharide biosynthesis.
Links with industry / industrial relevance (22)
The team collaborates closely, on an "open laboratory" basis with the industrial laboratories of Unilever plc, a major European food industry, at Bedford. I shall have the opportunity of visiting the Unilever laboratories, gaining exposure to the xyloglucan network model, to state of the an methodologies to measure cell wall rheology, and to an array of physicochemical method applied to plant cell walls.

Programme(s)

Programmes de financement pluriannuels qui définissent les priorités de l’UE en matière de recherche et d’innovation.

Thème(s)

Les appels à propositions sont divisés en thèmes. Un thème définit un sujet ou un domaine spécifique dans le cadre duquel les candidats peuvent soumettre des propositions. La description d’un thème comprend sa portée spécifique et l’impact attendu du projet financé.

Appel à propositions

Procédure par laquelle les candidats sont invités à soumettre des propositions de projet en vue de bénéficier d’un financement de l’UE.

Données non disponibles

Régime de financement

Régime de financement (ou «type d’action») à l’intérieur d’un programme présentant des caractéristiques communes. Le régime de financement précise le champ d’application de ce qui est financé, le taux de remboursement, les critères d’évaluation spécifiques pour bénéficier du financement et les formes simplifiées de couverture des coûts, telles que les montants forfaitaires.

RGI - Research grants (individual fellowships)

Coordinateur

University of Stirling
Contribution de l’UE
Aucune donnée
Adresse

FK9 4LA Stirling
Royaume-Uni

Voir sur la carte

Coût total

Les coûts totaux encourus par l’organisation concernée pour participer au projet, y compris les coûts directs et indirects. Ce montant est un sous-ensemble du budget global du projet.

Aucune donnée

Participants (1)

Mon livret 0 0