Objective
The objective of the proposed study is to determine the distribution of band 3-like protein (AE2) in the gills of aquatic vertebrates. The distribution of AE2 will be determined using immunocytochemistry at the light and electron microscopic levels. A suitable antibody probe will be developed against fish erythroid band 3. I will purify the cytoplasmic fragment of band 3 protein from teleost (Oncorhynchus mykiss) red blood cells under non denaturing conditions using differential centrifugation and ion exchange and size exclusion chromatography. Antibodies will be raised against this purified peptide and once the specificity has been validated used to determine the cellular distribution of AE2 in the fish gill under different environmental conditions. In this way the function of different cell types (acid, base excreting cells) in this acid-base reguatory epithelium can be determined.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
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Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
67037 Strasbourg
France
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