Immunocytochemical and biochemical characterization of fmrp in neurons

Project Information

Grant agreement ID: FMBI982879

Project closed

Start date 1 October 1998

End date 30 September 2000

Funded under

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Total cost No data

EU contribution No data

Coordinated by

Erasmus Universiteit Rotterdam
Netherlands

Objective

Research objectives and content
The fragile X syndrome is the most common form of inherited mental retardation in man and is caused by a dynamic mutation in the FMR-1 gene, that it is located in Xq27.3. The mutational basis of the syndrome is an expansion of a trinucleotide repeat (CGG) just upstream of coding region. As a result, transcription suppression through hypermethylation of the promoter region occurs. Lack of the expression of the gene product, FMRP, is responsible for the pathogenesis of the fragile X syndrome. Thus far the physiological funcion of FMRP is unknown. An understanding of the function of FMRP would provide insight into the molecular basis of the fragile X syndrome.The goal of this study is further characterization of FMRP in neurons using different experimental approaches.
Training content (objective, benefit and expected impact)
Using biotinylated antibodies against FMRP I will study the expression pattern of FMRP in normal and transgenic mice, with special emphasis to the central nervous system. At the subcellular level I will study the routing of FMRP, using cultured neurons which are transfected with a plasmid containing either wildtype or mutant forms of FMRP.In addition, biochemical studies will be performed to characterize tne different molecular forms and possible interaction(s) of FMRP with other proteins or RNA's. Links with industry / industrial relevance (22)

Fields of science (EuroSciVoc)

CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.

• natural sciences › biological sciences › neurobiology
• natural sciences › biological sciences › biochemistry › biomolecules › proteins
• natural sciences › biological sciences › genetics › mutation
• natural sciences › biological sciences › genetics › RNA

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Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Topic(s)

• 0302 - Post-doctoral research training grants
• TL05 - Cell Biology

Call for proposal

Data not available

Funding Scheme

RGI - Research grants (individual fellowships)

Coordinator

Erasmus Universiteit Rotterdam

• EU contribution: No data
• Address: 50,Dr. Molewaterplein ; 3015 GE Rotterdam ; Netherlands
• Total cost: No data