Objective
Research objectives and content:
Human KRAB zinc finger proteins (KRAB ZFPs) represent a large subfamily of ZFPs of the Kruppel Cys2His2 (C2H2)-type containing a Krueppel associated box (KRAB) domain in their amino termini. Based on the finding that the KRAB domain can silence transcription when fused to a heterologous DNA binding domain in transiently transfected mammalian cells, the KRAB ZFPs have been postulated to function as negative regulators of transcription in many levels of cellular growth and differentiation. However, there is also the possibility that they participate in more general functions concerning structure and organisation of chromosomes. Recently, two specific KRAB-interacting proteins have been identified and characterized: TIF1alpha and TIF1a, which both belong to the same gene family and
have many of the properties expected for corepressors acting at the chromatin level. TIF1alpha but not TIF1a was shown to interact directly with the ligand-dependent transcriptional activation function AF2 of nuclear receptors (NRs), indicating that some members of the TIF1 gene family may play a dual role in transcription, being involved in both repression by KRAB ZFPs and reactivation by agonist-bound NRs The aim of this project is to address the biological significance of the KRAB ZFP-TIF1 and TIF1-NR interactions.
Training content:
We will performed a yeast two hybrid screen using the KRAB-interacting domains of TIFlalpha and TIF1a as individual baits and a mouse embryo cDNA expression library. By this approach, mouse cDNAs
encoding KRAB ZFPs that interact specifically with TIF1alpha and/or TIF1a will be identified and characterized.
The corresponding genomic DNAs will be isolated for the
subsequent disruption of the individual genes in mouse. The consequence(s) of these null mutations on development, cell differentiation and gene induction by nuclear receptors will be investigated. In addition, an in vitro transcription and chromatin assembly system that supports KRAB-mediated repression will be established and investigated for hormone-dependent reactivation.
Links with industry/industrial relevance:
We expect the project to result in the identification of new genes encoding regulatory proteins, some of which might be associated with specific human diseases. Therefore, the project will provide the basis for the development of new diagnostic and therapeutic concepts.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics DNA
- natural sciences chemical sciences inorganic chemistry transition metals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics chromosomes
- medical and health sciences clinical medicine embryology
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
67404 ILLKIRCH GRAFFENSTADEN
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.