Comparison of regulatory sequences of polyhomeotic and its human homologs inchromatin activity in drosophila

Informations projet

N° de convention de subvention: FMBI983224

Projet clôturé

Date de début 21 Decembre 1998

Date de fin 20 Decembre 2000

Financé au titre de

• Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Coût total Aucune donnée

Contribution de l’UE Aucune donnée

Coordonné par

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
France

Objectif

Research objectives and content
Objective A: To develop improved site specific recombinase based strategies for targetted chromosomal integration of DNA in Drosophila. This will enable the problem of genomic position effects in the analysis of chromatin mediated regulation to be overcome.
Objective B: To use the above targetting strategy for the
comparison, at the same integration locus,of regulatory regions of the Drosophila gene polyhomeotic and its human homologs.The isolation and characterisation of higher eukaryotic homologs of Drosophila Polycomb group (PcG) and trithorax group (trxG) genes has shown that chromatin mediated mechanisms of silencing and activation appear to be broadly conserved throughout evolution. In Drosophila, the polyhomeotic gene, itself a member of the PcG, is regulated by PcG and trxG products. I propose an analysis of the regulatory regions of human homologs of po-eotic (HPHI and HPH2), and comparison with those of the Drosophila gene, based on assays in Drosophila for chromatin silencing and activation.
Training content (objective, benefit and expected impact)
I will learn techniques in Drosophila genetics and the analysis of chromatin activity in Drosophila. Benefit / impact This new training in combination with my experience in biochemistry and molecular biology, and my familiarity with site specific recombinase technology, will give me a powerful range of skills with which to study mechanisms of chromatin regulation.
Links with industry ! industrial relevance (22)

Champ scientifique (EuroSciVoc)

CORDIS classe les projets avec EuroSciVoc, une taxonomie multilingue des domaines scientifiques, grâce à un processus semi-automatique basé sur des techniques TLN.

• sciences naturelles › sciences biologiques › biochimie
• sciences naturelles › sciences biologiques › génétique › ADN
• sciences naturelles › sciences biologiques › biologie moléculaire

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Programme(s)

• FP4-TMR - Specific research and technological development programme in the field of the training and mobility of researchers, 1994-1998

Thème(s)

• 0302 - Post-doctoral research training grants
• TL06 - Developmental Biology

Appel à propositions

Data not available

Régime de financement

RGI - Research grants (individual fellowships)

Coordinateur

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE

• Contribution de l’UE: Aucune donnée
• Adresse: Rue de la Cardonille 141 ; 34396 MONTPELLIER ; France
• Coût total: Aucune donnée