Objective
Research objectives and content
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Potato virus X (PVX), type member of the potexviruses, uses an uncharacterized strategy for movement within its hosts. Filamentous virions are believed to move through plasmodesmata, cytoplasmic bridges that connect adjacent cells, aided by viral and host proteins. The PVX movement proteins appear to play a role different to that described for the movement of other viruses. Cell and Molecular Biological techniques will be applied to investigate the following topics. -The role of the plant cytoskeleton in intra- and inter-cellular movement of PVX in Mcotiana benthamiana plants. The cellular co-localization of fluorescently labeled viral particles and proteins with cell proteins and structures will be determined by fluorescence and confocal microscopy. -The role of viral proteins in the movement of PVX. The contribution of viral proteins to plasmodesmata gating will be determined by analyzing the ability of dextran dyes to pass through the plasmodesmata of cells infected with movement deficient PVX mutants. Electron microscopy will be used to investigate localization of viral proteins and the nature of . plasmodesmatal structural modification.. -The potential for interactions
between viral proteins will be tested biochemically and candidate host proteins involved in viral . interactions will be sought by probing a plant yeast two-hybrid library. .
Training content (objective, benefit and expected impact)
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The training in modern Cell Biology techniques as Confocal microscopy and Microinjection of dextran dyes will satisfy the real . need for a Cell Biologist in spanish outstanding Plant research centres. The importance of the Agriculture sector in Spain, as one . of the main crop producers in the European Union, make necessary an effort of the Institutions to train Plant Pathologists in the . modern techniques that will aid to the improvement of disease control measures and the consequent increase of the quality and . yield of the production. It is also important to note that understanding the basis of viral movement will help design novel . transgenic resistance strategies. .
Links with industry / industrial relevance (22).
Dr. Simon Santa Cruz has industrial contracts to develop PVX-based protein expression vectors. This project will render useful information to optimize the extent and rate of the movement of viruses used as protein expression vectors in plants.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences cell biology
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences physical sciences optics microscopy confocal microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
DD2 5DA Dundee
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.