Skip to main content
Ir a la página de inicio de la Comisión Europea (se abrirá en una nueva ventana)
español es
CORDIS - Resultados de investigaciones de la UE
CORDIS
Contenido archivado el 2024-04-30

The contribution of the actin cytoskeleton and a potential rho-gtpase to theroot-hair deformation during rhizobium infection

Objetivo



Research objectives and content
The symbiotic interaction of the bacterium Rhizobium with the root nodules of leguminous plants i crucial for nitrogen fixation. It has been shown that Nod-factors secreted by the bacteria are important for signaling the plant cells to initiate the early steps of nodulation. One of these steps is the deformation a root hairs. This morphological change is the result of re-establishing polar growth and is driven by changes in the actin cytoskeleton. In other systems, rho proteins play an important role in polar growth and in mechanisms controlling actin cytoskeleton configuration. The recent identification of a rho family protein in these plants in T. Bisseling's group suggests that this GTPase may regulate the actin cytoskeleton in this system. The aim of my proposal is to study the actin cytoskeleton in living root hair using actin-GFP constructs and the potential regulation of the actin configuration by the novel rho-protein in more detail. This project has two experimental goals. First, to visualize the actin cytoskeleton in living root hairs. Second, to overexpress the wild type and different mutants of the rho-protein and to correlate these conditions with a morphological and biochemical characterization of the actin cytoskeleton in root hairs.
Training content (objective, benefit and expected impact)
In Ton Bisseling's group I have the opportunity to learn a completely new subject (plant cell biology) as well as molecular biological methods. This project may give insights which are of general importance for signal: tansduction in plants.
Links with industry / industrial relevance (22)
Nitrogen fixation is a crucial process for Agriculture throughout the world.

Ámbito científico (EuroSciVoc)

Programa(s)

Tema(s)

Convocatoria de propuestas

Datos no disponibles

Régimen de financiación

RGI - Research grants (individual fellowships)

Coordinador

WAGENINGEN UNIVERSITY
Aportación de la UE
Sin datos
Dirección
3,Dreijenlaan 3
6703 HA WAGENINGEN
Países Bajos
Coste total
Sin datos