Objective
Research objectives and content
The existence of five highly conserved subunits that are common to all three RNA polymerases is one + the less well understood features of eukaryotic transcription. My project focusses on ABC27 (or Rpb5 is the only common subunit that is not functionally interchangeable between human and yeast. Recent evidence suggests that it communicates with transcriptional transactivators and thus directly contributes the regulation of gene expression. We propose to obtain a functional human ABC27 protein in yeast, by directed mutagenesis, by construction of chimeric proteins between human and yeast, and by direct searching for yeast suppressor mutants in which the human form of the subunit would be functional in vivo. Recent unpublished data (based on a two hybrid assay) strongly suggest that ABC27 interacts with conserved domain located on the largest subunit of the three yeast RNA polymerases. We shall examine there is a direct correlation between the ability to support growth and this interaction. This genetic a molecular approaches are meant to clarify the role played by ABC27/Rpb5 in eukariotic transcription complexes and, by extension, may help understanding the general role(s) played by the common subunits.
Training content (objective, benefit and expected impact)
the host institution is among the leading european laboratories in the field of eucaryotic transcription a yeast molecular biology. It will therefore provide high level training in a field that is not yet well develop in Spain. Working in the group of Dr. P. Thuriaux, will specifically introduce me to the molecular genetic of an eukaryotic organism, thereby completing my previous formation in the field of prokariotic molecular biology and genetics.All techniques and skill acquired will be of great interest to my future retour to Spain. Links with industry / industrial relevance (22)
The human form of RPB5 is thought to directly interact with the HBx protein of the human hepatitis B virus. Understanding the precise role of that subunit might therefore open new therapeutic perspectives for Hepatitis B, even though this is not the primary objective of our research. Similarly, the African Swine Fever Virus (a major veterinary plague) encodes an Rpb5-like protein that is in all likelyhood a component of its transcription machine. One outcome of our project will be to identify the most critical residues of ABC27, which are also present in the viral protein: this may conceivably be relevant to viral therapy.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- medical and health sciences health sciences infectious diseases
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics RNA
- natural sciences biological sciences molecular biology
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
91191 Gif-sur-Yvette
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.