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Content archived on 2024-05-14

Relating mitotic control to the mechanics of cell division

Objective



We will utilise the genetic, developmental, and cytological advantages of genetics, cell and developmental biology, with biochemistry. It thereby Drosophila to study the centrosome cycle and cytokinesis, both of which closely resemble analogous processes in mammalian cells. Mitotic entry provides an ideal training ground for a multidisciplinary approach to a fundamental biological problem. The collaborating laboratories each have requires the P34 kinase activated by cdc25. We will study the roles of two genes that we have identified that regulate a Drosophila cdc25 homologue, skills that span these experimental disciplines, although having some degree of specialisation so that collectively they can provide all of the one of which appears to affect centrosome behaviour. We will characterise mutations in the two Drosophila genes for gama-tubulin and identify genes necessary expertise and training to the proposed postdoctoral workers encoding proteins that interact with gama-tubulin within the centrosome.

Centrosome separation and spindle formation will be studied with reference to the phosphorylation- cycles of kinesin-like proteins and other centrosomal components by the polo kinase and protein phosphatase 2A. We will characterise several genes essential for cytokinesis, and study their interactions with reference to the localisation and phosphorylation of the gene products.

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Coordinator

Universidade do Porto
EU contribution
No data
Address
823,Rua do Campo Allegre
4100 Porto
Portugal

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Total cost

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Participants (5)

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