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STRUCTURE AND FUNCTION of PROTEINS INVOLVED IN SKELETAL MUSCLE EXCI TATION-CONTRACTION coupling

Objective



The eight research groups have established a wide spectrum of modern of skeletal muscle. Action potentials spreading along the transverse techniques such as i) biochemical identification and purification of proteins, ii) cloning, sequencing, site directed mutagenesis, and tubular membrane into the cell interior cause a conformational change of expression of genes, iii) immunocytochemical identification of proteins, the voltage-sensitive dihydropyridine receptors (DHPR) embedded in this iv) ultrastructural localisation of proteins, v) functional membrane. It is by a complex and not yet understood process that activated characterisation of proteins in pharmacological binding assays, vi) DHPR triggers the release of Ca2+ from internal Ca2+ stores, the characterisation of heterologously expressed or reconstituted channel sarcoplasmic reticulum (SR). Ca2+ is conducted by the ryanodine receptor (RyR) situated in the SR membrane into the cytoplasma where it activates proteins by patch and bilayer clamping.
the contractile apparatus. More than 10 proteins associated with DHPR and RyR, such as triadin, FKBP12, calsequestrin and several not yet identified proteins are required for functional EC coupling.
Here, eight experienced research groups will employ an integrated and multi-disciplinary approach to characterise known and novel proteins of the DHPR/RyR complex and their relationships. The major objective comprises the expression of normal and recombinant known proteins, the identification, isolation and expression of novel proteins, and the analysis of their interactions at structural and functional levels. This project will significantly advance our understanding of the role of the proteins involved in EC coupling.

Coordinator

Universität Ulm
Address

89069 Ulm
Germany

Participants (7)

Commissariat à l'Energie Atomique (CEA)
France
Address
Centre D'etudes De Grenoble
38041 Grenoble
Debrecen University of Medicine
Hungary
Address
98,Nagyerdei Krt 98
4012 Debrecen
LEOPOLD-FRANZENS-UNIVERSITAET INNSBRUCK
Austria
Address
1,Peter-mayr-strasse 1
6020 Innsbruck
UNIVERSITY COLLEGE CORK, NATIONAL UNIVERSITY OF IRELAND, CORK
Ireland
Address
Lee Maltings, Prospect Row
30 Cork
University College Dublin
Ireland
Address
Fosters Avenue Blackrock
4 Dublin
Università degli Studi di Ferrara
Italy
Address
Via Luigi Borsari 46
44100 Ferrara
Universität Ulm
Germany
Address

89069 Ulm