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A new method for the construction of a high resolution map of the whole human genome

Objective



Irradiation and fusion gene transfer (IFGT, also known as "radlation hybrids") has been used to construct high resolutlon genetic maps of defined regions of the human genome. The current methods for produclng IFGT maps have the drawback of requiring "single chromosome" hybrids as donors, furthermore, between 100 and 200 radiation hybrids are required for each human chromosome mapped. To construct a map of the whole genome upto 5,000 hybrid cell lines would be needed. The origlnal idea for IFGT technique was suggested by pontecorvo and was pioneered by Goos and Harris. Instead of "single chromosome hybrids" the latter authors used human cells as donors. We have repeated these early experiments and have found that non-selected markers are retalned at frequencies sufficiently high that the same set of hybrids can be used to map the whole genome. A set of 200 hybrids should be capable of generating a map with an average marker spacing of less than 500kb. Based on this observation and by combining the skills of the Cambridge laboratory in somatic cell genetics with the expertise of Genethon in marker testing, we propose to create a high resolution map of the whole human genome. This map will have different biases from the recombination map and will be particularly useful for constructing YAC contigs of the human genome. In the future, we propose to extend this technology to map the genomes of other large mammals.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

University of Cambridge
Address
Downing Street
CB2 3EH Cambridge
United Kingdom

Participants (1)

GENETHON III
France
Address
Rue De L'internationale 1Bis
91002 Evry