Objective
This project aims to better understanding of the molecular processes involved in carotid plaque morphology and seventy of vessel narrowing in the pathogenesis of ischaemic stroke associated with symptomatic and asymptomatic carotid stenosis. We propose to investigate the changes in gene activation, particularly those coding for angiogenic molecules and associated second messenger molecules in carotid plaques, serum and ischaemic brain following stroke. We will try to identify the link molecules between plaque pathology, serum cytokine expression and penumbra evolution in patients with symptomatic and asymptomatic carotid disease. Further we want to extend our observations in humans to in vitro and in vivo models. Beneficial or detrimental activation of each signal transduction pathway will be assessed on the base of cell survival and induction of apoptosis or necrosis in the carotid plaques and ischaemic penumbra.
The main objectives are the following:
1) Screening for changes in gene expression using Clontech Atlas multi-array technology. Important genes involved in signal transduction pathways will be identified and these will be studied in more detailes;
2) Immunohistochemistry, western blotting and protein activity studies for Fas, TNFRI, ras, raf, MAP-ERK family, PI3K, AKT, bad, Bclx-L;
3) Quantification of expression of the second messengers: PLC, PLA2, MAPK, and cytokines: VEGF, TGF, and PDGF families;
4) In the second year animal stroke model of focal cerebral ischaemia will be studied. The expression of the most important molecules will be compared with those found in humans;
5) Cell death mechanisms implicated in the plaque pathology will be studied. We will concentrate on the caspase-dependent and caspase-independent pathways. This will be compared to our initial studies in animal models.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences basic medicine pathology
- medical and health sciences basic medicine neurology stroke
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Data not available
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
08034 BARCELONA
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.